Production of antibody against aflatoxin B1.
نویسندگان
چکیده
Antibody against aflatoxin B1 was obtained after one multiple-site injection of bovine serum albumin-aflatoxin B1 conjugate into rabbits. The antibody has greatest binding efficiency for aflatoxin B1, less efficiency for B2, G1, and Q1, and least for aflatoxicol, G2, and M1. Sterigmatocystin, coumarin, and 4-hydroxycoumarin did not give a cross-reaction with the antibody. The sensitivity of the binding assay for detection of aflatoxin B1 is in the range of 0.2 to 2.0 ng per 0.5-ml sample. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody titer determination are described.
منابع مشابه
Five of 12 forms of vaccinia virus-expressed human hepatic cytochrome P450 metabolically activate aflatoxin B1.
Twelve forms of human hepatic cytochrome P450 were expressed in hepatoma cells by means of recombinant vaccinia viruses. The expressed P450s were analyzed for their abilities to activate the potent hepatocarcinogen aflatoxin B1 to metabolites having mutagenic or DNA-binding properties. Five forms, P450s IA2, IIA3, IIB7, IIIA3, and IIIA4, activated aflatoxin B1 to mutagenic metabolites as assess...
متن کاملکاهش عوارض آفلاتوکسیکوزیس تجربی در بلدرچین ژاپنی با استفاده از پادتن اختصاصی زرده تخم
Two experiments were carried out to determine the potential of oral yolk derived specific antibody against aflatoxin B1 in Japanese quails. In the first experiment egg yolk derived antibody against aflatoxin B1 was produced in layer quails. The potential of aflatoxin absorbent of the eggs from immunized and non-immunized layers was assayed in vitro. Egg yolk extracts derived from aflatoxin-BSA ...
متن کاملProduction and characterization of monoclonal antibodies for aflatoxin B1.
Hybridomas that secreted antibodies for aflatoxin B1 were selected using two immunization protocols referred to as A and B. Protocol A is a standard immunization method and resulted in the selection of only two clones that produced monoclonal antibodies against aflatoxin B1. In protocol B a unique immunization schedule which resulted in the generation of 10 hybridomas is described. Of the 10, o...
متن کاملProtective Effects of Bacillus sp. MBIA2.40 and Gallipro on Growth Performance, Immune Status, Gut Morphology and Serum Biochemistry of Broiler Chickens Feeding by Aflatoxin B1
A total of 540 one-day old male broilers Ross 308 were allocated to 6 treatments in a completely randomized designed with a 2×3 factorial arrangement (aflatoxin B1 (AFB1) and probiotics) with 6 replicates and 15 birds in each. Factors include aflatoxin B1 (control, 500 ppb) and probiotics (control, 108 cfu/mL Bacillus sp. MBIA2.40, 0.2 g Gallipro per kg). Results showed that adding 500 ppb AFB1...
متن کاملDevelopment of antibody-based microarray assay for quantitative detection of aflatoxin B1
BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metaboliteproduced by Aspergillus species that contaminates a wide range ofagricultural products. OBJECTIVES: This study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of AFB1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in fo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 33 5 شماره
صفحات -
تاریخ انتشار 1977